猪Slc22a18与Slc22a3的克隆及印迹表达分析

doi:10.11843/j.issn.0366-6964.2014.10.004

Abstract

Abstract:

To enrich the number of imprinted genes and to provide a basis for future imprinting research in swine，the candidate imprinted genes Slc22a18 and Slc22a3 were identified.In this study，657 bp cDNA sequence of Slc22a18 and 1 077 bp cDNA sequence of Slc22a3 cDNA were obtained and their imprinting status were identified by using a single nucleotide polymorphisms-based sequencing method in 16 tissues.Imprinting analysis showed that Slc22a18 was biallelically expressed in all the tissues of 1-month-old piglet and maternally expressed in the 45 d placenta.And the expression pattern of Slc22a3 prefered to maternal allele.The expression level of Slc22a18 was significantly different among tissues and higher in the liver，kidney and small intestine.Slc22a3 was widely expressed in various tissues.The results indicated that imprinting status of Slc22a18 and Slc22a3 were polymorphic in different tissues，and Slc22a18 and Slc22a3 were maternal expressed genes in pigs.

CLC Number:

S828
S813.3

ZHOU Yang，JIAO Ming-xia，LYU Jia-wei,ZHU Jiang，YU Miao，KONG Qing-ran，LIU Zhong-hua. The Cloning and Imprinting Analysis of Slc22a18 and Slc22a3 in Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1600-1607.

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References

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The Cloning and Imprinting Analysis of Slc22a18 and Slc22a3 in Swine

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